This activity requires that students work with bacterial cultures and open flames. Model proper laboratory safety practices for your students and require them to adhere to all laboratory safety rules. Use personal protective equipment such as safety glasses or goggles, gloves, and aprons when appropriate. Demonstrate the protocol for correctly using the instruments and materials necessary to complete the activities, and emphasize the importance of proper usage. See our Carolina™ Techniques for Studying Bacteria and Fungi manual for more details on the proper handling of tube cultures.Įnsure that students understand and adhere to safe laboratory practices when performing any activity in the classroom or lab. Also, each workstation would need a test tube rack to hold the tube culture. If using tube cultures, students have to flame the mouth of the culture tube when inserting the inoculating loop and after withdrawing it and recapping the tube. These instructions are written for the use of plate cultures however, the Introductory Bacterial Set is also available as tube cultures (154755). ![]() ![]() If using toluidine blue, increase the staining time to 2 minutes. Lens paper for cleaning oil-immersion lens after labīibulous paper (preferred) or paper towelsĪlthough crystal violet is recommended for this activity, several other stains can be used, including methylene blue, basic fuchsin, and toluidine blue. Microscopes with oil-immersion lenses and immersion oil Introductory Bacteria Set, Plate Cultures (154756)īacillus subtilis (rods), Micrococcus luteus (spheres), and Rhodospirillum rubrum (spirals)īeakers or cups with droppers for deionized water
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